| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.257001 |
| Chromosome: | chromosome 6 |
| Location: | 1010957 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g256000 | (1 of 1) PF15996 - Arginine/serine-rich protein PNISR (PNISR) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGATCTGACTGGCTGGCCAGCAATCGAGG |
| Internal bar code: | TTAGAGGAGCCGAGGAGTCAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 105 |
| LEAP-Seq percent confirming: | 98.6207 |
| LEAP-Seq n confirming: | 286 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTGGTGACCGTAATCCT |
| Suggested primer 2: | CGAATATGTGTATCGCCGTG |