| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.257004 |
| Chromosome: | chromosome 9 |
| Location: | 3389996 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388875 | (1 of 112) 2.7.11.25 - Mitogen-activated protein kinase kinase kinase / MLTK | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTCCTTGCCAGCCTAGTCCTTTGAACGG |
| Internal bar code: | GGTGGGTGCCTGGTGCGTCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1052 |
| LEAP-Seq percent confirming: | 99.7367 |
| LEAP-Seq n confirming: | 8713 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCCTCGATTTCTCGTCAC |
| Suggested primer 2: | TGCCAAGGTTGAGGATTTTC |