Insertion junction: LMJ.RY0402.257007_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.257007
Chromosome:chromosome 8
Location:2560687
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g372500 (1 of 1) K11373 - elongator complex protein 1 (ELP1, IKI3, IKBKAP) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTATCTACAACCCCCCTCCCCTCCCCTC
Internal bar code:TAGCTGCAGGCTAATTTTGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:305
LEAP-Seq percent confirming:83.3333
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CACACGGACACAAACACACA
Suggested primer 2:AGGAACGTCGCAAGTTGTCT