| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.257030 |
| Chromosome: | chromosome 12 |
| Location: | 6007180 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g535019 | (1 of 139) IPR029787 - Nucleotide cyclase | gene_edge/mRNA_edge/3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCCCTTTGCTTGTGACACATCGCAAGCA |
| Internal bar code: | ACGTTCTCGGGTGGTGTGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 464 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAAGCAGCGGCAGATACAT |
| Suggested primer 2: | GTTGCATGCTTCTGCGATAA |