Insertion junction: LMJ.RY0402.257038_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.257038
Chromosome:chromosome 3
Location:2270920
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g157950 (1 of 1) IPR011989//IPR011990//IPR016024 - Armadillo-like helical // Tetratricopeptide-like helical domain // Armadillo-type fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTAGAACTGAACTAGAACCGAAAACCGTCG
Internal bar code:AAGGTGCTGCCCGAACCTCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:662
LEAP-Seq percent confirming:99.3274
LEAP-Seq n confirming:443
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CATTGACACACCGACACACA
Suggested primer 2:TAGTTTCTCTCCGTGGCGTT