Insertion junction: LMJ.RY0402.257038_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.257038
Chromosome:chromosome 3
Location:2270923
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g157950 (1 of 1) IPR011989//IPR011990//IPR016024 - Armadillo-like helical // Tetratricopeptide-like helical domain // Armadillo-type fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAAACAAAGCACCGTGTGCCGTACTAATAA
Internal bar code:AGCATAATGATGGTTGGGCACG

Confirmation - LEAP-Seq

LEAP-Seq distance:586
LEAP-Seq percent confirming:99.5396
LEAP-Seq n confirming:8215
LEAP-Seq n nonconfirming:38
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CATTGACACACCGACACACA
Suggested primer 2:TAGTTTCTCTCCGTGGCGTT