| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.257059 |
| Chromosome: | chromosome 6 |
| Location: | 1979702 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g263950 | (1 of 1) 3.6.3.9 - Sodium/potassium-exchanging ATPase / Sodium/potassium-transporting ATPase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGCGTGAATACATCCACATGCGCCGCAC |
| Internal bar code: | CCTAGTACAGTATGAGACGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 471 |
| LEAP-Seq percent confirming: | 99.6062 |
| LEAP-Seq n confirming: | 10371 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTTCCTGTATGCTGCTCC |
| Suggested primer 2: | CTGGAATCCAATCCCAGCTA |