| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.257068 |
| Chromosome: | chromosome 10 |
| Location: | 3674051 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g445900 | (1 of 1) PF00226//PF14901 - DnaJ domain (DnaJ) // Cleavage inducing molecular chaperone (Jiv90) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGGTCATAGCTACACGCGTCACCTTGCG |
| Internal bar code: | AGTGAGCGTTTGTAGTAGAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 608 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 237 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGTACAGGGGTTGTTCTA |
| Suggested primer 2: | CTCCCTAATGCGGGTCTACA |