| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.257071 |
| Chromosome: | chromosome 17 |
| Location: | 3445714 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g724650 | (1 of 9) PTHR13789//PTHR13789:SF186 - MONOOXYGENASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACTCGGCCACCTACCGCTACGAGCTTTC |
| Internal bar code: | GTTGCCGCTCTGCTTCATCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 726 |
| LEAP-Seq percent confirming: | 99.1163 |
| LEAP-Seq n confirming: | 673 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCAGGTCACAAGGTGGAT |
| Suggested primer 2: | GCATCGCTACCTCTCACACA |