Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.257127 |
Chromosome: | chromosome 3 |
Location: | 8545301 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g201888 | (1 of 2) IPR027414 - Glycosyl hydrolase family, N-terminal domain | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGCAGGCAGCGGCTCCGGCTCTCGTGTG |
Internal bar code: | AGCGTCTGGTGTCCTGTCCGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 465 |
LEAP-Seq percent confirming: | 99.373 |
LEAP-Seq n confirming: | 634 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCAAACACCCAAGCATTTCT |
Suggested primer 2: | GCTCCATAGAAGCGCCATAG |