| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.257149 |
| Chromosome: | chromosome 12 |
| Location: | 6913731 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g560650 | (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGGTGGTCTGGTCGGCACGCACCGGCG |
| Internal bar code: | CGGTTTTATGCGGAGAGCATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 144 |
| LEAP-Seq percent confirming: | 50.9524 |
| LEAP-Seq n confirming: | 214 |
| LEAP-Seq n nonconfirming: | 206 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTCGCTAACCTCGATTAC |
| Suggested primer 2: | TCTAGGAAACGGCCAAAATG |