Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.257156 |
Chromosome: | chromosome_16 |
Location: | 3090387 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g665450 | FAP48,RYR1 | Ryanodine-inositol 1,4,5-triphosphate receptor Ca2+ channel | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCGGCCACGCGTTGCACCTCCATGACCTGC |
Internal bar code: | GCACGTTGTGCGCACGCCTAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 553 |
LEAP-Seq percent confirming: | 99.3841 |
LEAP-Seq n confirming: | 1291 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCACGCAGGGTATAAGGGTA |
Suggested primer 2: | TGTCCAAGCGCTGAGTTATG |