| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.257165 |
| Chromosome: | chromosome 10 |
| Location: | 4854889 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g454300 | (1 of 1) IPR003613//IPR011009 - U box domain // Protein kinase-like domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGGAACAATCAAGCTGGCCAGTTTGTCC |
| Internal bar code: | AAAGGGTTTTCGCTACTAAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 158 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 71 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAGCTTCTGCCTCATAAC |
| Suggested primer 2: | CGTGTGTCTTTTGCCGACTA |