Insertion junction: LMJ.RY0402.257171_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.257171
Chromosome:chromosome 2
Location:3877960
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g096000 (1 of 14) 3.6.3.25 - Sulfate-transporting ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGGTGAGGGTCACTGGGGCGGAGGGTGAA
Internal bar code:AGGTCCGGGGAAACTCGCCAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:503
LEAP-Seq percent confirming:98.5075
LEAP-Seq n confirming:1650
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:TTCCGTTTGACCATCCTCTC
Suggested primer 2:CTTGTTCCGGTGTTGATCCT