| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.257199 |
| Chromosome: | chromosome 17 |
| Location: | 3272430 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g723000 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGCACCAAATGCGGTTTCTTTGCGCAGC |
| Internal bar code: | ATAAGGGGCAGAAGCCCGGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 527 |
| LEAP-Seq percent confirming: | 98.0488 |
| LEAP-Seq n confirming: | 603 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCAGCAGCTGCATTACCT |
| Suggested primer 2: | CCAATAGGCGCAGTTAGAGC |