| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.257202 |
| Chromosome: | chromosome 16 |
| Location: | 2367231 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g659850 | CGL37 | conserved protein related to shikimate kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGACGCGGATGTCATGTTGGGCATGTGT |
| Internal bar code: | TCCGGGTCTGCAAATCGTGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1166 |
| LEAP-Seq percent confirming: | 97.835 |
| LEAP-Seq n confirming: | 6462 |
| LEAP-Seq n nonconfirming: | 143 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGGTGATAAGCAAATGTT |
| Suggested primer 2: | GCTGCTTTTGCTCACCCTAC |