| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.257202 |
| Chromosome: | chromosome 16 |
| Location: | 2367232 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g659850 | CGL37 | conserved protein related to shikimate kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCATTCACCACCAGGCATTAACATCGCCC |
| Internal bar code: | GTCGATAGAGGTACACCCGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 543 |
| LEAP-Seq percent confirming: | 94.8776 |
| LEAP-Seq n confirming: | 2908 |
| LEAP-Seq n nonconfirming: | 157 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGGTGATAAGCAAATGTT |
| Suggested primer 2: | GCTGCTTTTGCTCACCCTAC |