| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.257210 |
| Chromosome: | chromosome 10 |
| Location: | 969008 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g424750 | PPD1 | (1 of 2) 2.7.9.1 - Pyruvate, phosphate dikinase / Pyruvate,phosphate dikinase; Pyruvate phosphate dikinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCGCGCGCGCGGTTCTCCTTCTGCGTG |
| Internal bar code: | CAGGCATTCAGTTTGCCGAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 212 |
| LEAP-Seq percent confirming: | 99.4924 |
| LEAP-Seq n confirming: | 196 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGGAGAGCAGATGTGATA |
| Suggested primer 2: | TAACGTTGAACCAATCCCGT |