| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.257232 |
| Chromosome: | chromosome 11 |
| Location: | 780967 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467636 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGGTCTGAGCGGACGACTAGACGTGGAG |
| Internal bar code: | CAGGCACACGTTTATCGTTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 609 |
| LEAP-Seq percent confirming: | 98.7608 |
| LEAP-Seq n confirming: | 797 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTGGCACGGATAAGTACG |
| Suggested primer 2: | ATATGGCAATCTGCAGGGTC |