| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.257234 |
| Chromosome: | chromosome 12 |
| Location: | 1343693 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g488752 | (1 of 70) 4.6.1.1 - Adenylate cyclase / ATP pyrophosphate-lyase | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCGGACAAGATGCGTAAGCACACGAATG |
| Internal bar code: | CATCGGGATTCCACCGTGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 99 |
| LEAP-Seq percent confirming: | 99.2337 |
| LEAP-Seq n confirming: | 259 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACTGCAAAAGCTACCGGA |
| Suggested primer 2: | GCAGACGTGTTGATGTACCG |