| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.257253 |
| Chromosome: | chromosome 9 |
| Location: | 430343 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g404550 | (1 of 2) 2.7.11.20 - [Elongation factor 2] kinase / EF2K | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTATGTTTCATCGGGATGCGGCAGCGAACC |
| Internal bar code: | CCGGTGGCTTGCGTCTCTTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 98 |
| LEAP-Seq percent confirming: | 69.1729 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCTGTCACGATACCCCGT |
| Suggested primer 2: | CAGGCACTGCATGACAAAAC |