| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.257255 |
| Chromosome: | chromosome 13 |
| Location: | 3192479 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g585500 | HAP1 | (1 of 12) IPR000326 - Phosphatidic acid phosphatase type 2/haloperoxidase; Putative haloperoxidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGGGGCGGGTGATCAAACATGAAGCCCC |
| Internal bar code: | GACTCATTGTGATTCGTGCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 671 |
| LEAP-Seq percent confirming: | 98.8954 |
| LEAP-Seq n confirming: | 1343 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGATACGTTGCATTCACA |
| Suggested primer 2: | CTACGGTCTTGGGTTTGCAT |