Insertion cassette: | pMJ013b |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.SG0182.000160 |
Chromosome: | chromosome 16 |
Location: | 5521258 |
Confidence (%): | 75 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre16.g680500 | EFH10 | (1 of 38) IPR002048//IPR011992 - EF-hand domain // EF-hand domain pair; EF-hand Calcium binding protein, may be centrin-related | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGTGAGACCGGAGGACGG |
Internal bar code: | |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 812 |
LEAP-Seq percent confirming: | 95.0 |
LEAP-Seq n confirming: | 57 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGCAGGTCTAGCTGATGTGG |
Suggested primer 2: | GTGCAGTTGTGAAAGCGTGT |