Insertion junction: LMJ.SG0182.000177_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.000177
Chromosome:chromosome 12
Location:5526914
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre12.g531200 FOX2 Multicopper ferroxidase; (1 of 2) K14735 - hephaestin (HEPH) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGGCTGTGAGGTTGACCCC
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:505
LEAP-Seq percent confirming:84.3373
LEAP-Seq n confirming:70
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:CGAAGCGCTTTTAACTGGTC
Suggested primer 2:GTCCGTGATAGAGAGCCAGC