Insertion junction: LMJ.SG0182.000351_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:-
Strain:LMJ.SG0182.000351
Chromosome:chromosome 16
Location:1480418
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre16.g652850 ALG5,GTR4 Dolichyl-phosphate beta-glucosyltransferase; (1 of 1) 2.4.1.117 - Dolichyl-phosphate beta-glucosyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGCTTAGTACGGTGCGCACG
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:742
LEAP-Seq percent confirming:72.7586
LEAP-Seq n confirming:211
LEAP-Seq n nonconfirming:79
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GAAACGGGGAGTGAATCTGA
Suggested primer 2:TCTGATGTCACGGTTGGTGT