Insertion junction: LMJ.SG0182.000381_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:-
Strain:LMJ.SG0182.000381
Chromosome:chromosome 11
Location:2789169
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre11.g477400 CYG43 (1 of 1) PF00072//PF00211//PF02518 - Response regulator receiver domain (Response_reg) // Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc) // Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c); Adenylate/guanylate cyclase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAGACACGGAAACGGCTCA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:548
LEAP-Seq percent confirming:99.1837
LEAP-Seq n confirming:243
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GCCCAAATTAAACCAAACCA
Suggested primer 2:GGTGGTCAGGTAGGTCTCCA