Insertion junction: LMJ.SG0182.000458_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.000458
Chromosome:chromosome 12
Location:3593853
Confidence (%):75
Locus systematic id Locus common name Defline Feature
g12685 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTTCTCCCCATGCAAATGG
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:796
LEAP-Seq percent confirming:99.0291
LEAP-Seq n confirming:102
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGACCCGAACCAGTACAT
Suggested primer 2:GAAAAGACGGCAGAAACAGC