| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.SG0182.000510 |
| Chromosome: | chromosome 2 |
| Location: | 6201387 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g113850 | PBGD2,HMBS,HEM3 | (1 of 1) PTHR11557//PTHR11557:SF0 - PORPHOBILINOGEN DEAMINASE // PORPHOBILINOGEN DEAMINASE; Porphobilinogen deaminase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCGAGGGTGGGAGGAAG |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1210 |
| LEAP-Seq percent confirming: | 97.3904 |
| LEAP-Seq n confirming: | 2687 |
| LEAP-Seq n nonconfirming: | 72 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGATCAAGCACTCCTTCC |
| Suggested primer 2: | GCACACACACACACCCCTAC |