Insertion junction: LMJ.SG0182.000541_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.000541
Chromosome:chromosome 3
Location:1258493
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre03.g150000 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGATGTCTGCATCCTCTTCT
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:647
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCTGAGCGTCTCGTTTTC
Suggested primer 2:AACTCCCCGTCAACAACAAG