Insertion junction: LMJ.SG0182.000721_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.000721
Chromosome:chromosome 12
Location:7005975
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre12.g559850 (1 of 1) K12880 - THO complex subunit 3 (THOC3) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGAACACATAGACACAGAAC
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:876
LEAP-Seq percent confirming:78.5714
LEAP-Seq n confirming:66
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:TTCTGAAAGCAGAAGGGGAA
Suggested primer 2:TGCATACCGTATCAAGGCAA