Insertion junction: LMJ.SG0182.000957_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.000957
Chromosome:chromosome 1
Location:7522187
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre01.g054150 NTRC1,NTR4 (1 of 1) PTHR10438//PTHR22912//PTHR22912:SF48 - THIOREDOXIN // DISULFIDE OXIDOREDUCTASE // NADPH-DEPENDENT THIOREDOXIN REDUCTASE 3; NADPH-dependent thioredoxin reductase C, chloroplastic CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGTCAAAGGTCTCCGGCGT
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:600
LEAP-Seq percent confirming:78.2609
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTTCTGGGCTAGTGCAAC
Suggested primer 2:TGGGAAGCAAGGATCAAGAC