| Insertion cassette: | pMJ013b |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.SG0182.001362 |
| Chromosome: | chromosome_16 |
| Location: | 1629124 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g654300 | RSP23 | Radial spoke protein 23 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCAAGGGACGAGAAGGTGAGC |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 565 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTATCGCCAAGCAGAAGC |
| Suggested primer 2: | GCTGTCGGGATACACGAACT |