| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.SG0182.001447 |
| Chromosome: | chromosome 9 |
| Location: | 276705 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| g9144 | FMG1-B | Flagella membrane glycoprotein, major form | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTTCATGGTAACGTTTCAG |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 657 |
| LEAP-Seq percent confirming: | 76.0417 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCTCAACGTCACCTACA |
| Suggested primer 2: | TCAGCAGGCTACATGCATTC |