Insertion junction: LMJ.SG0182.001471_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:-
Strain:LMJ.SG0182.001471
Chromosome:chromosome 14
Location:876539
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre14.g613450 CGL87 (1 of 1) PTHR12818:SF0 - NEF-ASSOCIATED PROTEIN 1; Conserved in the Green Lineage intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTGCAGGGAGGGGGGGGG
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:897
LEAP-Seq percent confirming:83.3333
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:ACCACTACCACCACCACCAT
Suggested primer 2:CCCTGTGCACAACTTACCCT