Insertion cassette: | pMJ013b |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.SG0182.001909 |
Chromosome: | chromosome 9 |
Location: | 5715988 |
Confidence (%): | 75 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
g9996 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGGCACTGGCACTGGCGT |
Internal bar code: | |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1405 |
LEAP-Seq percent confirming: | 94.4713 |
LEAP-Seq n confirming: | 2153 |
LEAP-Seq n nonconfirming: | 126 |
LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGTGTATGTGTGATGCACCG |
Suggested primer 2: | GTAGTGGACTCTTCTGGCGG |