Insertion junction: LMJ.SG0182.002069_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.002069
Chromosome:chromosome 2
Location:6352651
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre02.g115350 (1 of 2) 2.7.7.59 - [Protein-PII] uridylyltransferase / Uridylyl removing enzyme intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTCCATTTCCGCTTCCGGT
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1375
LEAP-Seq percent confirming:99.3464
LEAP-Seq n confirming:456
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:TCATGTCTGGCACTCGTAGC
Suggested primer 2:ACGCTGCTCAACTATTGGCT