| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.SG0182.002170 |
| Chromosome: | chromosome 10 |
| Location: | 4706359 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g453600 | DES,FAD13 | (1 of 2) PTHR19353:SF30 - FATTY ACID DESATURASE 2; Fatty acid omega 13 desaturase | MULTIPLE_SPLICE_VARIANTS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAATCGTGCAATTCAAGTGTC |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1540 |
| LEAP-Seq percent confirming: | 99.9557 |
| LEAP-Seq n confirming: | 2258 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACAGCGCCGAGTCATACTG |
| Suggested primer 2: | TGCATGGTGTTTGTGGAAGT |