Insertion junction: LMJ.SG0182.002295_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:-
Strain:LMJ.SG0182.002295
Chromosome:chromosome 3
Location:8121219
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre03.g200800 CYG61 (1 of 11) 4.6.1.1//4.6.1.2 - Adenylate cyclase / ATP pyrophosphate-lyase // Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGATCTGATCTTCGCGTGCTG
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:823
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:97
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:CTTAGCGGCAAGAGTGGAAC
Suggested primer 2:TTTAGGGGTTTACCACGCTG