| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.SG0182.002356 |
| Chromosome: | chromosome 9 |
| Location: | 291263 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| g9144 | FMG1-B | Flagella membrane glycoprotein, major form | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACGCCGCCGGCGACCAGCT |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1266 |
| LEAP-Seq percent confirming: | 99.9167 |
| LEAP-Seq n confirming: | 3598 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAATGTGTCCGTGACCAG |
| Suggested primer 2: | GCAACAACATTCACCACGTC |