Insertion junction: LMJ.SG0182.002520_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:-
Strain:LMJ.SG0182.002520
Chromosome:chromosome 16
Location:5743909
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre16.g678700 (1 of 133) IPR012336 - Thioredoxin-like fold 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTCATGTACAATAGCCTAC
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:554
LEAP-Seq percent confirming:95.4545
LEAP-Seq n confirming:63
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTCTTTGCATGCGAGTGT
Suggested primer 2:TCATCCGTGTGTCAGAGGAC