Insertion junction: LMJ.SG0182.002614_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.002614
Chromosome:chromosome 11
Location:1492585
Confidence (%):75
Locus systematic id Locus common name Defline Feature
g11673 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGACTCAGTAACAGGCAG
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1122
LEAP-Seq percent confirming:97.4
LEAP-Seq n confirming:487
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCGCTCCTTCAAAACAAG
Suggested primer 2:CCACCAACTGCAACACATTC