Insertion cassette: | pMJ013b |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.SG0182.002784 |
Chromosome: | chromosome 10 |
Location: | 2968615 |
Confidence (%): | 75 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g440550 | (1 of 14) PF13540 - Regulator of chromosome condensation (RCC1) repeat (RCC1_2) | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACTGCGCGGCGTCATGAC |
Internal bar code: | |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 532 |
LEAP-Seq percent confirming: | 93.75 |
LEAP-Seq n confirming: | 330 |
LEAP-Seq n nonconfirming: | 22 |
LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTCCCCTGTCCACAACATCT |
Suggested primer 2: | GGCTTCTGCCTTATCGACAC |