Insertion junction: LMJ.SG0182.002801_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.002801
Chromosome:chromosome 13
Location:1153642
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre13.g569600 (1 of 5) PF03992 - Antibiotic biosynthesis monooxygenase (ABM) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGTAGGATGCGGCGCATGTG
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:526
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:72
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GAAACCCAGAGTTGGGATGA
Suggested primer 2:GATACTAAGGCAATCGGGCA