Insertion junction: LMJ.SG0182.002820_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.002820
Chromosome:chromosome 6
Location:2665350
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre06.g270100 SBE2a,SBE2 (1 of 3) 2.4.1.18 - 1,4-alpha-glucan branching enzyme / Glycogen branching enzyme; Starch Branching Enzyme 2 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACTGGATGGACGAGTTCAA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:774
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:151
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:AGTAGACACGGGGAGTGTGG
Suggested primer 2:CACAATGCCGTCCTTATCCT