| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.SG0182.002846 |
| Chromosome: | chromosome 12 |
| Location: | 6959507 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g560300 | MULTIPLE_SPLICE_VARIANTS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGGCCCCCCGCCTCATCAT |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1108 |
| LEAP-Seq percent confirming: | 87.7551 |
| LEAP-Seq n confirming: | 215 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAACGCGACTTTACAGGGC |
| Suggested primer 2: | CTGCTGAGAAGACACCACCA |