| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.SG0182.002904 |
| Chromosome: | chromosome 5 |
| Location: | 2565182 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g236050 | (1 of 2) IPR015097 - Lung surfactant protein D coiled-coil trimerisation | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCCTCGCCCAGGCGCTGCA |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1420 |
| LEAP-Seq percent confirming: | 97.6823 |
| LEAP-Seq n confirming: | 2866 |
| LEAP-Seq n nonconfirming: | 68 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTGACAGCTCAGATTGC |
| Suggested primer 2: | GGCCTCACCGTGTATGACTT |