Insertion junction: LMJ.SG0182.002980_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:-
Strain:LMJ.SG0182.002980
Chromosome:chromosome 10
Location:5691842
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre10.g460600 CYN53 (1 of 2) PTHR11071//PTHR11071:SF297 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE // CYCLOPHILIN-LIKE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FAMILY PROTEIN; Cyclophilin 53 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACATTCCTTCTGGACCCCGTC
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1346
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:203
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:TGGTCACCCAACCAGTACCT
Suggested primer 2:ACAAACACACAGAGGGAGGG