Insertion junction: LMJ.SG0182.003001_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:-
Strain:LMJ.SG0182.003001
Chromosome:chromosome 2
Location:3444354
Confidence (%):75
Locus systematic id Locus common name Defline Feature
g1888 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCCGCGGCTGCGGAACAG
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:757
LEAP-Seq percent confirming:98.5916
LEAP-Seq n confirming:70
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTGTGTGTGTGTGTGTGT
Suggested primer 2:TCGAAGCACCACTCATTCTG