Insertion junction: LMJ.SG0182.003110_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.003110
Chromosome:chromosome 2
Location:7333481
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre02.g145150 MAM3A (1 of 5) K16302 - metal transporter CNNM (CNNM); Protein putatively involved in mitochondrial biogenesis MULTIPLE_SPLICE_VARIANTS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAGGTGCGGCTGTGTGTGGG
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:684
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:61
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GTTGTCTCTGGAGGAGACGC
Suggested primer 2:CTCCTTGTGGTGGCTATGGT