Insertion junction: LMJ.SG0182.003156_2

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:-
Strain:LMJ.SG0182.003156
Chromosome:chromosome 1
Location:4399190
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre01.g029800 Coiled-Coil Protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAGCCCGGCGCTACTGAAGA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:811
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:81
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:TTGCTGAACGTGAAGCACTC
Suggested primer 2:AAACAGTATGAACGGACGCC